KMID : 0352720170410020188
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Journal of Ginseng Research 2017 Volume.41 No. 2 p.188 ~ p.194
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Preventive effect of fermented black ginseng against cisplatin-induced nephrotoxicity in rats
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Jung Ki-Won
An Jun-Min Eom Dae-Woon Kang Ji-Sung Kim Su-Nam
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Abstract
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Background: Fermented black ginseng (FBG) is processed ginseng by the repeated heat treatment and fermentation of raw ginseng. The protective effect and mechanism of FBG on cisplatin-induced nephrotoxicity was investigated to evaluate its therapeutic potential.
Methods: The free radical scavenging activity of FBG was measured using 1,1-diphenyl-2-picrylhydrazyl (DPPH). In addition, the protective effect against cisplatin-induced renal damage was tested in rats. FBG was orally administered every day at a dose of 150 mg/kg body weight for 10 d, and a single dose of cisplatin was administered intraperitoneally (7.5 mg/kg body weight) with 0.9% saline on the 4th d.
Results: The DPPH radical-scavenging activity of FBG (IC50=384 ¥ìg/mL) was stronger than that of raw ginseng. The improved DPPH radical-scavenging activity was mediated by the generation phenolic compounds. The decreased cell viability by cisplatin was recovered significantly after treatment with FBG in a dose-dependent manner. Then, the protective effect of FBG on cisplatin-induced oxidative renal damage was investigated in rats. The decreased creatinine clearance levels, which are a reliable marker for renal dysfunction in cisplatin-treated rats, were reduced to the normal level after the administration of FBG. Moreover, FBG showed protective effects against cisplatin-induced oxidative renal damage in rats through the inhibition of NF-¥êB/p65, COX-2, and caspase-3 activation.
Conclusion: These results collectively show that the therapeutic evidence for FBG ameliorates the nephrotoxicity via regulating oxidative stress, inflammation, and apoptosis.
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KEYWORD
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cisplatin, fermented black ginseng, oxidative stress, Panax ginseng, renal damage
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